CD86 antibody (PE)

Catalog No. ABIN6935779

This anti-CD86 antibody is a Mouse Monoclonal antibody detecting CD86 in FACS, IF, BP and T. Suitable for Human, Mouse and Rat.

Quick Overview for CD86 antibody (PE) (ABIN6935779)

Target: CD86

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD86 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Blocking Peptide (BP), Test (T)

Clone: BU63

Product Details anti-CD86 Antibody (PE)

Specificity: Recognizes a protein of 70 kDa, which is identified as CD86 (HLDA V, WS Code BP BP072. HLDA V, WS Code A A109. HLDA VI, WS Code BP 95. HLDA VI, WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.

Purification: Purified by Protein A/G

Immunogen: ARH-77 (B-lymphoblastoid cell line)

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: 293T or Jukat cells. Monocytes and dendritic cells, activated T, B and natural killer cells in lymph node or tonsil.

Known Application: Flow Cytometry: 5 µL per test per one million cells (or 5 µL per 100 µL of whole blood)Immunofluorescence (1:50-1:100)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD86

Target: CD86

Alternative Name: CD86

Molecular Weight: 70kDa

Gene ID: 942

UniProt: P42081

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation