CD34 antibody (CF®488A)

Catalog No. ABIN6935795

This Mouse Monoclonal antibody specifically detects CD34 in FACS, IF and T. It exhibits reactivity toward Human and Rat.

Quick Overview for CD34 antibody (CF®488A) (ABIN6935795)

Target: CD34

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD34 antibody is conjugated to CF®488A

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Test (T)

Clone: ICO-115

Product Details anti-CD34 Antibody (CF®488A)

Specificity: This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85 % of angiosarcoma and Kaposi's sarcoma, but shows low specificity.

Purification: Purified by Protein A/G

Immunogen: Blast cells of a chronic myeloid leukemia patient

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: KG-1 cells. Human tonsil or angiosarcoma.

Known Application: Flow Cytometry: 5 µL per test per one million cells (or 5 µL per 100 µL of whole blood)Immunofluorescence (1:50-1:100)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD34

Target: CD34

Alternative Name: CD34

Molecular Weight: 90-110kDa

Gene ID: 947

UniProt: P28906