Recombinant ACP5 antibody

Catalog No. ABIN6936344

This Rabbit Monoclonal antibody specifically detects ACP5 in IHC (f). It exhibits reactivity toward Human.

Quick Overview for Recombinant ACP5 antibody (ABIN6936344)

Target: ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ACP5 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: ACP5-2336R

Product Details anti-ACP5 Antibody

Specificity: It recognizes a protein of 35kDa, which is identified as tartrate-resistant acid phosphatase (TRAcP). It exists as two isoforms (5a and 5b). This MAb reacts with both the isoforms. Serum TRAcP 5a is secreted by macrophages and dendritic cells and increased in many patients of rheumatoid arthritis.Serum TRAcP 5b is produced from osteoclasts and elevated during bone resorption. TRAcP is an iron containing glycoprotein, which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. TRAcP is synthesized as a latent proenzyme and is activated by proteolytic cleavage and reduction. Normally, TRAcP is highly expressed by osteoclasts, activated macrophages, neurons and endometrium during pregnancy. Expression of TRAcP is increased in certain pathological conditions such as Leukemic Reticuloendotheliosis (Hairy Cell Leukemia), Gaucher's Disease, HIV-induced Encephalopathy, Osteoclastoma and in osteoporosis and metabolic bone diseases. Anti-TRAcP antibody labels the cells of Hairy Cell Leukemia (HCL) with a high degree of sensitivity and specificity. Other cells stained with this antibody are tissue macrophages and osteoclasts.

Cross-Reactivity (Details): Human. Mouse. Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human ACP5 protein

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HepG2, 293T, K562 or RPMI-8226 cells. Spleen from Hairy Cell Leukemia (HCL) patient.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ACP5

Target: ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))

Alternative Name: ACP5

Background: Acid phosphatase 5, tartrate resistant, ACP5, serum band 5 tartrate-resistant acid phosphatase, SPENCDI, Tartrate-resistant acid ATPase, Tartrate-resistant acid phosphatase type 5, TR-AP, TRACP5, TRAcP, TRAP, TrATPase, Type 5 acid phosphatase,TRAcP (Tartrate-Resistant Acid Phosphatase) (Hairy Cell Leukemia Marker)
Cellular localisation: Cytoplasmic (Lysosomes)

Molecular Weight: 35kDa

Gene ID: 54, 1211

UniProt: P13686

Pathways: Transition Metal Ion Homeostasis