ACPP antibody

Catalog No. ABIN6936347

This Mouse Monoclonal antibody specifically detects ACPP in IF, FACS and IHC (f). It exhibits reactivity toward Human.

Quick Overview for ACPP antibody (ABIN6936347)

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACPP antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: PASE-4LJ

Product Details anti-ACPP Antibody

Specificity: Recognizes a protein of 52 kDa, identified as prostate specific acid phosphatase (PSAP). This enzyme catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland.PSAP is found in non-neoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas. It shows no staining in granulocytes, osteoclasts, parietal cells of the stomach, liver cells, renal cell or breast carcinomas.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Prostatic acid phosphatase Purified from human seminal plasma

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: PC12 cells. Normal prostate or prostate carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ACPP

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Alternative Name: ACPP

Background: 5'-nucleotidase (5'-NT), Acid phosphatase prostate, ACP3, Ecto-5'-nucleotidase, Prostatic acid phosphatase (PAP), Prostatic acid phosphatase, Thiamine monophosphatase (TMPase),Prostate Specific Acid Phosphatase (PSAP)
Cellular localisation: Cytoplasmic

Molecular Weight: 52kDa

Gene ID: 55, 433060

UniProt: P15309

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process