APC antibody (AA 1-433)

Catalog No. ABIN6936423

The Mouse Monoclonal anti-APC antibody has been validated for WB, ELISA, IF, FACS and Coat. It is suitable to detect APC in samples from Human.

Quick Overview for APC antibody (AA 1-433) (ABIN6936423)

Target: APC (Adenomatous Polyposis Coli (APC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat)

Clone: ALi 12-28

Product Details anti-APC Antibody

Binding Specificity: AA 1-433

Specificity: The adenomatous polyposis syndromes, familial adenomatous polyposis (FAP) and Gardner's syndrome (GS), are characterized by numerous adenomatous polyps throughout the entire colon. These polyps invariably progress to colon cancer in addition to other extracolonic manifestations. The cloning of the APC gene revealed a ubiquitously expressed protein, 2,843 amino acids in length, which is frequently mutated in patients suffering from FAP and GS. APC has been found to be associated with structural components of intracellular junctions. Functions as a tumor suppressor, promoting rapid degradation of CTNNB1 and is a negative regulator of Wnt signaling. Also plays a role in HGF-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.

No Cross-Reactivity: Mouse (Murine)

Cross-Reactivity (Details): Human. Does not react with Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human APC protein (around aa 1-433)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: COLO 320DM whole cell lysate. HCT116 cells. Tonsil, placenta, breast or colon.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for APC

Target: APC (Adenomatous Polyposis Coli (APC))

Alternative Name: APC

Background: Adenomatous Polyposis Coli, CC1, Deleted in polyposis 2.5 (DP2.5), DP2, DP3, FAP, FPC, GS, Protein APC,APC / Adenomatous Polyposis Coli / FAP (Tumor Suppressor)
Cellular localisation: Cell membrane and cytoplasm.

Molecular Weight: 66kDa

Gene ID: 324, 158932

UniProt: P25054

Pathways: WNT Signaling, Stem Cell Maintenance, Cell-Cell Junction Organization