BrdU antibody

Catalog No. ABIN6936516

The Mouse Monoclonal anti-BrdU antibody has been validated for IHC, FACS, IF, Neut and StM. It is suitable to detect BrdU in samples from .

Quick Overview for BrdU antibody (ABIN6936516)

Target: BrdU (Bromodeoxyuridine (BrdU))

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This BrdU antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Neutralization (Neut), Staining Methods (StM)

Clone: SPM166

Product Details anti-BrdU Antibody

Specificity: It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Purification: Purified by Protein A/G

Immunogen: Bromodeoxyuridine (BrdU) conjugated to KLH

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Use Ab at 1 μg/mL for 30 minute at RT)[For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1 mg/mL PBS, 10 min at 37oC Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Buffer: 10 mM PBS without BSA and without Azide.

Preservative: Azide free

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Expiry Date: 24 months

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Target Type: Chemical

Molecular Weight: 307Da