CD3 epsilon antibody

Catalog No. ABIN6936692

This Mouse Monoclonal antibody specifically detects CD3 epsilon in FACS, IF, Act and ProA. It exhibits reactivity toward Human.

Quick Overview for CD3 epsilon antibody (ABIN6936692)

Target: CD3 epsilon (CD3E)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Activation (Act), Proliferation Assay (ProA)

Clone: CRIS-7

Product Details anti-CD3 epsilon Antibody

Specificity: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Cross-Reactivity (Details): Human. Monkey.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Stimulated human leukocytes

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Induce T cell activation and proliferation (Order Ab without Azide),Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat cells. Tonsil and lymph node.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Background: CD 3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex,CD3e (T-Cell Marker)
Cellular localisation: Cell surface and cytoplasmic

Molecular Weight: 20kDa

Gene ID: 916, 3003

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway