CD3 epsilon antibody

Catalog No. ABIN6936700

This anti-CD3 epsilon antibody is a Mouse Monoclonal antibody detecting CD3 epsilon in FACS, IHC, IF, Func and IHC (fro). Suitable for Human.

Quick Overview for CD3 epsilon antibody (ABIN6936700)

Target: CD3 epsilon (CD3E)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: OKT3

Product Details anti-CD3 epsilon Antibody

Specificity: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. This MAb has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection.

Cross-Reactivity (Details): Human. Others not known.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human PBMC s

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (0.5-1 μg/million cells), ,Immunohistochemistry (acetone fixed frozen sections), ,Immunofluorescence (1-2 μg/mL), ,Functional Studies (Order Ab without Azide),Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat cells or PBMCs. Tonsil and lymph node

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Background: CD3 epsilon, CD3 TCR complex, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E,CD3e (T-Cell Marker)
Cellular localisation: Cell surface and cytoplasmic

Molecular Weight: 23kDa

Gene ID: 916, 3003

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway