CD5 antibody

Catalog No. ABIN6936749

This Mouse Monoclonal antibody specifically detects CD5 in FACS, IF, IP and Func. It exhibits reactivity toward Human.

Quick Overview for CD5 antibody (ABIN6936749)

Target: CD5

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD5 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Functional Studies (Func)

Clone: CRIS-1

Product Details anti-CD5 Antibody

Specificity: Recognizes a 67 kDa transmembrane protein, which is identified as CD5 (HLDA I, WS Code T 29HLDA III, WS Code T 530). The CD5 antigen is found on 95 % of thymocytes and 72 % of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10 %) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms.

Cross-Reactivity (Details): Human. Rhesus Monkey.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Stimulated human leukocytes

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Functional Studies (Order Ab without BSA & Azide), Immunoprecipitation (0.5-1 μg/500 μg protein lysate), Optimal dilution for a specific application should be determined.

Positive_Control: 293T, Ramos or MOLT-4 cells. Tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD5

Target: CD5

Alternative Name: CD5

Background: CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5,CD5 (Mantle Cell Lymphoma Marker)
Cellular localisation: Cell surface

Molecular Weight: 67kDa

Gene ID: 921, 58685

UniProt: P06127