Recombinant CD6 antibody

Catalog No. ABIN6936770

This Rabbit Monoclonal antibody specifically detects CD6 in FACS, IF and IHC (f). It exhibits reactivity toward Human.

Quick Overview for Recombinant CD6 antibody (ABIN6936770)

Target: CD6

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD6 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: C6-2884R

Product Details anti-CD6 Antibody

Specificity: CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular scavenger receptor cysteine-rich (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human recombinant full-length CD6 protein

Isotype: IgG

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MOLT-4, CCRF-CEM, Jurkat cells, Tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD6

Target: CD6

Alternative Name: CD6

Background: T12, TP120,CD6 (Negative Marker of T-regulatory Cells)
Cellular localisation: Cell Surface and Cytoplasmic

Molecular Weight: 90-130kDa

Gene ID: 923, 744366

UniProt: P30203