CD63 antibody (AA 100-197)

Catalog No. ABIN6936780

The Mouse Monoclonal anti-CD63 antibody has been validated for WB, IHC, ISt and IHC (f). It is suitable to detect CD63 in samples from Human.

Quick Overview for CD63 antibody (AA 100-197) (ABIN6936780)

Target: CD63

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD63 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: LAMP3-2788

Product Details anti-CD63 Antibody

Binding Specificity: AA 100-197

Specificity: This MAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Cross-Reactivity (Details): Human and Mouse. Others not known.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human CD63 protein fragment (around aa100-197) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Western Blotting (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Human spleen or lymphoma tissue (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD63

Target: CD63

Alternative Name: CD63

Background: Gp55, granulophysin, Lysosomal-associated membrane protein 3 (LAMP-3), Mast cell antigen AD1, Melanoma-associated antigen ME491, MLA1, NGA, Ocular melanoma-associated antigen, OMA81H, PTLGP40, Tetraspanin-30, TSPAN30,CD63 (Late Endosomes Marker)
Cellular localisation: Cell Surface. Cytoplasm.

Molecular Weight: 26kDa (core protein), 30-60kDa (glycosylated)

Gene ID: 967, 445570

UniProt: P08962