Recombinant CD81 antibody

Catalog No. ABIN6936837

The Rabbit Monoclonal anti-CD81 antibody has been validated for FACS and IHC (f). It is suitable to detect CD81 in samples from Human.

Quick Overview for Recombinant CD81 antibody (ABIN6936837)

Target: CD81

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD81 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: C81-2885R

Product Details anti-CD81 Antibody

Specificity: Recognizes a protein of 26 kDa, identified as CD81 (Workshop VI, Code CD81.1)). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Cross-Reactivity (Details): Human, Mouse and Rat. Others not known.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: B-Cell line derived from a Burkitt lymphoma

Isotype: IgG

Application Details

Application Notes:

Known_Application: Flow Cytometry (0.5-1 μg/million cells), Immunohistology (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, Ramos, Daudi, Raji, U266, HUT-78, HUT-102, Jurkat, HL-60, KG1a, A293, A431, MG63, and human lymphocytes. Tonsil or Liver.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD81

Target: CD81

Alternative Name: CD81 / TAPA-1

Background: CD81, Target of the antiproliferative antibody 1 (TAPA-1), Tetraspanin-28, Tspan-28,CD81 / TAPA-1
Cellular localisation: Cell surface

Molecular Weight: 26kDa

Gene ID: 975, 54457

UniProt: P60033

Pathways: Inositol Metabolic Process, Hepatitis C