Cadherin-16 antibody (AA 371-507)

Catalog No. ABIN6936887

This Mouse Monoclonal antibody specifically detects Cadherin-16 in ELISA, IHC (f) and Coat. It exhibits reactivity toward Human.

Quick Overview for Cadherin-16 antibody (AA 371-507) (ABIN6936887)

Target: Cadherin-16 (CDH16)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cadherin-16 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Clone: CDH16-2448

Product Details anti-Cadherin-16 Antibody

Binding Specificity: AA 371-507

Specificity: This MAb recognizes a protein of 130 kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels.Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 371-507) of human CDH16 protein (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order Ab without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Normal kidney or renal cell carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Cadherin-16

Target: Cadherin-16 (CDH16)

Alternative Name: CDH16

Background: Cadherin-16 (CDH16), Kidney-specific cadherin, Ksp-cadherin antibody,Ksp-Cadherin / CDH16 (Renal Cell Marker)
Cellular localisation: Cell Surface and Cytoplasmic

Molecular Weight: 130kDa

Gene ID: 1014, 513660

UniProt: O75309