Chromogranin A antibody

Catalog No. ABIN6936985

This anti-Chromogranin A antibody is a Mouse Monoclonal antibody detecting Chromogranin A in WB and IHC (f). Suitable for Human.

Quick Overview for Chromogranin A antibody (ABIN6936985)

Target: Chromogranin A (CHGA)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Chromogranin A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CHGA-777

Product Details anti-Chromogranin A Antibody

Specificity: Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.

No Cross-Reactivity: Rat (Rattus)

Cross-Reactivity (Details): Human. Does not react with Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human CHGA protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: PC12 cells. Adrenal gland, bowel, thyroid, pancreas or pheochromocytoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Chromogranin A

Target: Chromogranin A (CHGA)

Alternative Name: CHGA

Background: Beta-Granin, CGA, CHGA, Chromogranin A Parathyroid Secretory Protein 1, ER-37, Pancreastatin, Parastatin, Pituitary Secretory Protein I, SP-I, Vasostatin I or II,Chromogranin A / CHGA (Neuroendocrine Marker)
Cellular localisation: Finely granular cytoplasmic

Molecular Weight: 68-75kDa

Gene ID: 1113, 150793

UniProt: P10645

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling