CPS1 antibody

Catalog No. ABIN6937017

The Mouse Monoclonal anti-CPS1 antibody has been validated for IHC (f). It is suitable to detect CPS1 in samples from Human.

Quick Overview for CPS1 antibody (ABIN6937017)

Target: CPS1 (Carbamoyl-Phosphate Synthase 1, Mitochondrial (CPS1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CPS1 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CPS1-1022

Product Details anti-CPS1 Antibody

Specificity: This MAb recognizes a protein of 165kDa, identified as carbamoyl phosphate synthetase 1 (CPS1). This mitochondrial enzyme catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells.Deficiency of CPS1 is an autosomal recessive disorder that causes hyperammonemia. CPS1 is a hepatocyte specific protein that localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC's occur primarily in the stomach, but they are also found in many other organs. CPS1 may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of CPS1 correlates with smaller tumor size and longer patient survival. Occasionally, CPS1 is also found in gastric carcinomas as well as in a few other non-hepatic tumors.

Cross-Reactivity (Details): Human. Dog.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human CPS1 protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Liver or Hepatocellular Carcinoma (HCC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CPS1

Target: CPS1 (Carbamoyl-Phosphate Synthase 1, Mitochondrial (CPS1))

Alternative Name: CPS1

Background: Carbamoyl-phosphate synthetase 1 (CPS1), Carbamoylphosphate synthetase 1, CPSase 1, CPSASE1,CPS1 / Carbamoyl-Phosphate Synthetase (Hepatocellular Marker)
Cellular localisation: Finely granular cytoplasmic

Molecular Weight: ~165kDa

Gene ID: 1373, 149252

UniProt: P31327

Pathways: Response to Growth Hormone Stimulus, Cellular Glucan Metabolic Process