CYP1A1/2 antibody

Catalog No. ABIN6937070

This Mouse Monoclonal antibody specifically detects CYP1A1/2 in WB, IF and IHC (f). It exhibits reactivity toward Rat.

Quick Overview for CYP1A1/2 antibody (ABIN6937070)

Target: CYP1A1/2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1/2 (CYP1A1/2))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CYP1A1/2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: MC1

Product Details anti-CYP1A1/2 Antibody

Specificity: Cytochrome P450 oxidase (commonly abbreviated CYP) is a generic term for a large number of related, but distinct, oxidative enzymes important in vertebrate physiology. The cytochrome P450 mixed-function monooxygenase system is probably the most important element of Phase I metabolism in mammals. P450s are membrane-bound, either in the inner membrane of mitochondria or in the endoplasmic reticulum of cells where they metabolise thousands of endogenous and exogenous compounds. In the liver, these substrates include toxins, drugs, and other unneeded and potentially harmful molecules. Humans have 18 families of cytochrome P450 genes and 43 subfamilies, the CYP1 family is involved in drug metabolism and includes 3 subfamilies, 3 genes and 1 pseudogene.

Cross-Reactivity (Details): Human. Mouse. Rat. Primate.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: 3-methylcholanthrene induced rat cytochrome P450 protein.

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Liver or Cardiac muscle.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CYP1A1/2

Target: CYP1A1/2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1/2 (CYP1A1/2))

Alternative Name: CYP1A1 / CYP1A2

Background: Aryl hydrocarbon hydroxylase, CP11, CP12, CYP1A1, CYP1A2, CYPIA1, CYPIA2, Cytochrome P450 family 1 subfamily A polypeptide 1, Cytochrome P450 family 1 subfamily A polypeptide 2, P1 450, P3 450, P450 form 4, P450 form 6, P450 P1, P450 P3,Cytochrome P450 1A1/1A2
Cellular localisation: Cytoplasmic

Molecular Weight: 58kDa

Gene ID: 1543, 1544, 437060

UniProt: P04798, P05177