pan Keratin antibody

Catalog No. ABIN6937078

This anti-pan Keratin antibody is a Mouse Monoclonal antibody detecting pan Keratin in IF, FACS, WB, IHC and StM. Suitable for Human, Rat, Mouse, Cow, Pig, Frog, Guinea Pig, Goat and Marmoset.

Quick Overview for pan Keratin antibody (ABIN6937078)

Target: pan Keratin (panKRT)

Reactivity: Human, Rat, Mouse, Cow, Pig, Frog, Guinea Pig, Goat, Marmoset

Host: Mouse

Clonality: Monoclonal

Conjugate: This pan Keratin antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: SPM583

Product Details anti-pan Keratin Antibody

Specificity: This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Purification: Purified by Protein A/G

Immunogen: Keratin-enriched preparation from cultured human A431

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: A431 cells, Skin, Colon carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-4 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Buffer: 10 mM PBS without BSA and without Azide.

Preservative: Azide free

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Expiry Date: 24 months

Target Details for pan Keratin

Target: pan Keratin (panKRT)

Alternative Name: Cytokeratin, Multi

Gene ID: 3851, 3852, 3853

UniProt: P02538, P04259, P13647, P19013, P48668