Recombinant Cytokeratin, Multi (Epithelial Marker) antibody

Catalog No. ABIN6937080

The Rabbit Monoclonal anti- antibody has been validated for IHC, StM, FACS, IF and WB. It is suitable to detect in samples from Cow, Frog, Goat, Guinea Pig, Human, Monkey, Mouse, Pig and Rat.

Quick Overview for Recombinant Cytokeratin, Multi (Epithelial Marker) antibody (ABIN6937080)

Target: Cytokeratin, Multi (Epithelial Marker)

Antibody Type: Recombinant Antibody

Reactivity: Cow, Frog, Goat, Guinea Pig, Human, Monkey, Mouse, Pig, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Clone: KRT-1877R

Product Details

Specificity: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13) and 45 kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Purification: Purified by Protein A/G

Immunogen: Keratin-enriched preparation from cultured human epithelial cells

Isotype: IgG

Application Details

Application Notes:

Positive Control: A431 cells, Skin, Colon carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Buffer: 10 mM PBS without BSA and without Azide.

Preservative: Azide free

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Expiry Date: 24 months

Target Details

Target: Cytokeratin, Multi (Epithelial Marker)

Gene ID: 3851, 3852, 3853

UniProt: P02538, P04259, P13647, P19013, P48668