Desmoglein 1 antibody (Intracellular)

Catalog No. ABIN6937106

This Mouse Monoclonal antibody specifically detects Desmoglein 1 in WB, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Desmoglein 1 antibody (Intracellular) (ABIN6937106)

Target: Desmoglein 1 (DSG1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmoglein 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 18D4

Product Details anti-Desmoglein 1 Antibody

Binding Specificity: Intracellular

Specificity: Recognizes a protein of ~150 kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family.Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms.Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human desmoglein-1 intracellular domain

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL) Optimal dilution for a specific application should be determined.

Positive_Control: A431 or A-375 cells. Skin.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Desmoglein 1

Target: Desmoglein 1 (DSG1)

Alternative Name: DSG1

Background: Cadherin family member 4, CDHF4, Desmoglein-1, Desmosomal glycoprotein 1, DG1, DSG1, EPKHE, EPKHIA, Pemphigus foliaceus antigen, PPKS1, SPPK1,Desmoglein-1 (DSG1)
Cellular localisation: Cell surface

Molecular Weight: 150kDa (precursor), 160kDa (mature)

Gene ID: 1828, 2633

UniProt: Q02413

Pathways: Cell-Cell Junction Organization