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EGFR antibody

EGFR Reactivity: Human FACS, IP, AP, ProA, in vivo, Act Host: Mouse Monoclonal B1D8 unconjugated

Catalog No. ABIN6937132

Datasheet as PDF

Target See all EGFR Antibodies

EGFR (Epidermal Growth Factor Receptor (EGFR))
Reactivity
750

296

283

25

25

24

19

14

8

3

2

2

1

1

1

1

1

1
Human
Host
580

167

14

13

5

4

1

1
Mouse
Compatible Secondaries
Clonality
542

238

2
Monoclonal
Conjugate
412

58

29

22

19

17

16

16

16

16

16

11

10

10

10

10

10

10

10

10

10

8

8

5

4

4

4

4

4

1

1

1

1

1

1
This EGFR antibody is un-conjugated
Application
536

252

149

144

127

102

97

93

82

48

37

34

34

18

10

8

6

5

4

4

3

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
Flow Cytometry (FACS), Immunoprecipitation (IP), Affinity Purification (AP), Proliferation Assay (ProA), In vivo Studies (in vivo), Activation (Act)

Specificity

This antibody reacts with the extracellular domain of EGFR and blocks the EGF/TGF'induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin.

No Cross-Reactivity

Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details)

Human. Does not react with Mouse or Rat.

Purification

1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen

Microsomes from A431 cells.

Clone

B1D8

Isotype

IgG2a kappa

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EGFR Reactivity: Human WB, IF, IP Host: Rabbit Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibody
EGFR Reactivity: Human, Rat WB, ELISA, IHC, IP Host: Rabbit Polyclonal unconjugated

Application Notes

Known_Application: Affinity Purification (For coupling, order Ab without BSA), Blocks Ligand-induced Activation of EGFR, Inhibits Proliferation of A431 cells (Order Ab without sodium azide), Arrests Tumor Growth in vivo (Order Ab without BSA &Azide), Immunoprecipitation (1-2 μg/500 μg protein lysate), Flow Cytometry (1-2 μg/million cells), Optimal dilution for a specific application should be determined.
Positive_Control: A431 cells, Breast or Bladder cancer.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative

Azide free

Storage

-20 °C,-80 °C

Storage Comment

Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date

24 months
Target

EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name

EGFR (EGFR Products)

Background

Erbb1, ERBB1, Errp, HER1, mENA, PIG61, Proto-oncogene c-ErbB-1, Receptor Tyrosine Protein Kinase, ErbB1, Urogastrone, wa2, Wa5,EGFR (Epidermal Growth Factor Receptor)
Cellular localisation: Cell surface

Molecular Weight

~170kDa (wild type) and ~145kDa (vIII variant)

Gene ID

1956, 488293

UniProt

P00533

Pathways

NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins