Emerin antibody (AA 56-167)

Catalog No. ABIN6937146

The Mouse Monoclonal anti-Emerin antibody has been validated for WB and IHC (f). It is suitable to detect Emerin in samples from Human.

Quick Overview for Emerin antibody (AA 56-167) (ABIN6937146)

Target: Emerin (EMD)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Emerin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: EMD-2168

Product Details anti-Emerin Antibody

Binding Specificity: AA 56-167

Specificity: Emerin is a member of the nuclear lamina associated protein family. It is ubiquitously expressed and localized to the nuclear membrane in normal cells. Mutations of the gene that encodes emerin result in the X-linked recessive disease Emery-Dreifuss muscular dystrophy (EDMD), which is characterized by slowly progressing contractures, skeletal muscle wasting and cardiomyopathy. Reportedly, lack of Emerin expression is one cause of EDMD. Emerin is involved in the association of the nuclear membrane with the lamina, and is localized specifically to desmosomes and fasciae adherents in the heart. Identification of nuclear membrane irregularities with anti-emerin antibody has been reported useful in diagnosing papillary thyroid carcinoma.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human Emerin protein fragment (around aa 56-167) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: K-562, HeLa or Jurkat cells. Kidney or Skin.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Emerin

Target: Emerin (EMD)

Alternative Name: EMD

Background: EMD, Emerin, Emery Dreifuss muscular dystrophy (EDMD), STA,Emerin (Papillary Thyroid Carcinoma and EDMD Marker)
Cellular localisation: Nuclear Membrane

Molecular Weight: 37kDa

Gene ID: 2010, 522823

UniProt: P50402