ErbB2/Her2 antibody (Intracellular)

Catalog No. ABIN6937178

This Mouse Monoclonal antibody specifically detects ErbB2/Her2 in IF, ELISA, FACS and Coat. It exhibits reactivity toward Human.

Quick Overview for ErbB2/Her2 antibody (Intracellular) (ABIN6937178)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Coating (Coat)

Clone: ERB2-776

Product Details anti-ErbB2/Her2 Antibody

Binding Specificity: Intracellular

Specificity: This MAb is specific to c-erbB-2/HER-2 and shows minimal cross-reaction with other members of the family. C-erbB-2/HER-2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant intracellular domain of human HER-2 protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, SKBR-3 cells. Breast Cancers.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: ERBB2

Background: P185, CD340, Verb b2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog,HER-2 / c-erbB-2 / neu / CD340
Cellular localisation: Cell Surface

Molecular Weight: 185kDa

Gene ID: 2064, 446352

UniProt: P04626

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development