FCGR3A antibody

Catalog No. ABIN6937225

The Mouse Monoclonal anti-FCGR3A antibody (Clone ICO-116) (ABIN6937225) specifically detects FCGR3A in FACS and IF. The antibody is reactive with Human samples.

Quick Overview for FCGR3A antibody (ABIN6937225)

Target: FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FCGR3A antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: ICO-116

Product Details anti-FCGR3A Antibody

Specificity: It recognizes CD16 (FcgammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. Two similar genes represent CD16, CD16A (FcgammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (FcgammaRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human CD16 protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: K-562 or U-937 cells. Lymph nodes and tonsils.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for FCGR3A

Target: FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))

Alternative Name: FCGR3A

Background: FcRIIIa; FCR-10; FcRIII; FCGRIII; FcR-10; Fc fragment of IgG, low affinity IIIa, receptor for (CD16); neutrophil-specific antigen NA; CD16a; low affinity immunoglobulin gamma Fc region receptor III-A; Fc-gamma RIIIa; immunoglobulin G Fc receptor III; Fc-gamma RIII-alpha; Fc-gamma receptor IIIb (CD16); CD16a antigen; Fc-gamma receptor III-2 (CD16); FCGR3; Fc gamma receptor III-A; FCG3; low affinity III, receptor for (CD16); Fc-gamma RIII; IGFR3FcgammaRIIIA; CD16FCRIIIA; FCRIII; Fc fragment of IgG, low affinity IIIa, receptor (CD16a),CD16 / FcγReceptor III
Cellular localisation: Cell Surface

Molecular Weight: 50-80kDa

Gene ID: 2214, 372679

UniProt: P08637