FITC antibody

Catalog No. ABIN6937229

This Mouse Monoclonal antibody specifically detects FITC in WB, IHC, FACS, IF and CM.

Quick Overview for FITC antibody (ABIN6937229)

Target: FITC (Fluorescein Isothiocyanate (FITC))

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This FITC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Confocal Microscopy (CM)

Clone: SPM395

Product Details anti-FITC Antibody

Specificity: This MAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxy-fluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. In biotin or enzyme-labeled form, this MAb is also suitable for the conversation of fluorescein-labeled detection into immuno-enzymatic assays.

Purification: Purified by Protein A/G

Immunogen: Fluorescein isothiocyanate (FITC) conjugated to KLH

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Fluoresceinated proteins in solution or on tissues.

Known Application: Confocal Microscopy, Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), In situ Nucleic Acid Hybridization, Western Blot, Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Pretreatment depends upon the fluoreceinated primary antibody.)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Buffer: 10 mM PBS without BSA and without Azide.

Preservative: Azide free

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Expiry Date: 24 months

Target Details for FITC

Target: FITC (Fluorescein Isothiocyanate (FITC))

Alternative Name: FITC (Fluorescein Isothiocyanate)

Target Type: Chemical

Molecular Weight: 389.4Da (Molecular Formula: C21H11NO5S)