Fascin antibody

Catalog No. ABIN6937264

This anti-Fascin antibody is a Mouse Monoclonal antibody detecting Fascin in WB, FACS, IF and IHC (f). Suitable for Human.

Quick Overview for Fascin antibody (ABIN6937264)

Target: Fascin (FSCN1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fascin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: FSCN1-416

Product Details anti-Fascin Antibody

Specificity: Recognizes a protein of 55kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin's disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Full length recombinant human FSCN1 protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 8.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: K562, HeLa, MCF-7, PC-3 or BEWO cells. Hodgkin's lymphoma, Ovarian or Testicular Carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Fascin

Target: Fascin (FSCN1)

Alternative Name: FSCN1

Background: 55 kDa actin-bundling protein, FAN1, Fascin homolog 1, Fascin1, FSCN1, HSN, p55, Singed (Drosophila) like (sea urchin fascin homolog like), SNL,Fascin-1 (Reed-Sternberg Cell Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 55kDa

Gene ID: 6624, 118400

UniProt: Q16658