GPX4 antibody

Catalog No. ABIN6937347

The Mouse Monoclonal anti-GPX4 antibody has been validated for ELISA, IF, FACS, Coat and IHC (f). It is suitable to detect GPX4 in samples from Human.

Quick Overview for GPX4 antibody (ABIN6937347)

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GPX4 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: LHM 2

Product Details anti-GPX4 Antibody

Specificity: Glutathione Peroxidase 4 (GPX4) stabilizes cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. NG2 may facilitate primary melanoma progression by enhancing the activation of key signaling pathways important for tumor invasion and growth. Threonine 2256 phosphorylation of rat NG2 (threonine 2252 phosphorylation of human NG2) leads to redistribution of NG2 on the surface of astrocytomas, polarization of the cell and a significant increase in cell motility. NG2 acts as a co-receptor for spreading and focal contact formation in association with 1 integrin in malignant melanoma cells. NG2 is present on blood vessels throughout the rat embryo. Microvessels within the rat CNS express NG2 on endothelial cells, and outside the CNS, NG2 is present on smooth muscle cells. NG2 is a novel marker for epidermal stem cells that contributes to their patterned distribution by promoting stem cell clustering.

Cross-Reactivity (Details): Human. Mouse. Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A 375P cells crude extract

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF7 or HepG2 cells. Human brain or malignant melanoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for GPX4

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Alternative Name: GPX4

Background: Glutathione Peroxidase 4 (GPX4), Melanoma-associated Chondroitin Sulfate Proteoglycan (MCSP), Phospholipid hydroperoxidase (PHGPx), Sperm nucleus glutathione peroxidase (snGPx),GPX4 / MCSP
Cellular localisation: Mitochondrion. Cytoplasm.

Molecular Weight: 21kDa

Gene ID: 2879, 433951

UniProt: P36969