Recombinant CD235a/GYPA antibody

Catalog No. ABIN6937356

The Mouse Monoclonal anti-CD235a/GYPA antibody has been validated for WB and IHC (f). It is suitable to detect CD235a/GYPA in samples from Human.

Quick Overview for Recombinant CD235a/GYPA antibody (ABIN6937356)

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD235a/GYPA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: RGYPA-280

Product Details anti-CD235a/GYPA Antibody

Specificity: Recognizes a sialoglycoprotein of 39 kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human glycophorin A protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Erythrocytes in any human tissues. K562 cell lysate.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD235a/GYPA

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Alternative Name: GYPA

Background: Blood group--MN locus, GPA, GPErik, GpMiIII, GPSAT, GYPA, MN sialoglycoprotein, MNS, PAS2, Sialoglycoprotein alpha,Glycophorin A / CD235a (Erythrocyte Marker)
Cellular localisation: Cell surface

Molecular Weight: 39kDa

Gene ID: 2993, 2994, 434973

UniProt: P02724

Pathways: Maintenance of Protein Location