HLA-A antibody

Catalog No. ABIN6937390

This anti-HLA-A antibody is a Mouse Monoclonal antibody detecting HLA-A in FACS, IF and IHC (f). Suitable for Human.

Quick Overview for HLA-A antibody (ABIN6937390)

Target: HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-A antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CATA-1

Product Details anti-HLA-A Antibody

Specificity: This MAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31, B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This MAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2, DRw10

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: THP-1 and Jurkat cells. Tonsil or lymph node.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for HLA-A

Target: HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))

Alternative Name: HLA-A

Background: HLA-A, HLA class 1A, MHC class I HLA-A,HLA-Aw32 & HLA-A25 (MHC I)
Cellular localisation: Cell Surface

Molecular Weight: ~41kDa

Gene ID: 3105, 181244, 654404

UniProt: P01889, P30443, P30499

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response