GRP94 antibody

Catalog No. ABIN6937437

The Rat Monoclonal anti-GRP94 antibody is suitable to detect GRP94 in samples from Chicken. It has been validated for WB, FACS, IF and IHC (f).

Quick Overview for GRP94 antibody (ABIN6937437)

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivity: Chicken

Host: Rat

Clonality: Monoclonal

Conjugate: This GRP94 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 9G10-F8-2

Product Details anti-GRP94 Antibody

Specificity: Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp's are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

Cross-Reactivity (Details): Human. Monkey. Horse. Cow. Sheep. Pig. Rabbit. Dog. Rat. Mouse. Hamster. Guinea Pig. Chicken. Xenopus laevis.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Purified glucose regulated protein 94 (grp94) from chicken oviducts

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HepG2, U20S cells. About 50 % of breast tumors express grp94 protein.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Alternative Name: HSP90B1

Background: Glucose Regulated Protein 94, 94 kDa glucose-regulated protein, Endoplasmin, Endothelial cell (HBMEC) glycoprotein (ECGP), Heat shock protein 90 kDa beta member 1 (HSP90B1), Stress inducible tumor rejection antigen GP96, TRA1, Tumor rejection antigen-1 (gp96),GRP94 / HSP90B1 (Endoplasmic Reticulum Marker)
Cellular localisation: Cytoplasmic and nuclear

Molecular Weight: 94kDa

Gene ID: 7184, 192374

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades