ICAM-3/CD50 antibody

Catalog No. ABIN6937465

This anti-ICAM-3/CD50 antibody is a Mouse Monoclonal antibody detecting ICAM-3/CD50 in IF, IHC (f) and Bind. Suitable for Human.

Quick Overview for ICAM-3/CD50 antibody (ABIN6937465)

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM-3/CD50 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Binding Studies (Bind)

Clone: 186-2G9

Product Details anti-ICAM-3/CD50 Antibody

Specificity: Recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Stimulated human leukocytes

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Blocks binding of CD11a (LFA-1) to CD50 (ICAM-3) (Order Ab without Azide),Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HL-60 or THP-1 cells. Lymph node and tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ICAM-3/CD50

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Alternative Name: ICAM3

Background: ICAMR, Intercellular adhesion molecule 3 (ICAM3),CD50 / ICAM3
Cellular localisation: Cell surface

Molecular Weight: 110-160kDa

Gene ID: 3385, 354563

UniProt: P32942