ICOSLG antibody (AA 23-149)

Catalog No. ABIN6937473

The Mouse Monoclonal anti-ICOSLG antibody has been validated for WB, ELISA, IHC, FACS, IF, Coat and IHC (f). It is suitable to detect ICOSLG in samples from Human.

Quick Overview for ICOSLG antibody (AA 23-149) (ABIN6937473)

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICOSLG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: ICOSL-3111

Product Details anti-ICOSLG Antibody

Binding Specificity: AA 23-149

Specificity: This MAb recognizes a protein of 36 kDa, identified as ICOS-L. It is a ligand for the ICOS receptor that initiates T and B cell proliferation and cytokine secretion. ICOS-L interactions play an essential role in T cell-dependent B cell activation in peripheral lymphoid organs such as spleen and lymph nodes. ICOS-L protein is present in myeloid leukocytes, and by Northern blot there are 2.4, 3.0, and 7.0 kb transcripts in brain, heart, kidney, and liver, with lower expression in colon and thymus, and a 1.1 kb transcript in leukocytes. Tumor necrosis factor alpha (TNF), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) enhance its expression. LPS-induced up-regulation of ICOS-L is dependent on the MyD88-dependent signaling pathway.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human ICOSLG (B7RP-1) protein (around aa 23-149) (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: U937, HL60, HuT78 and HUVEC lysates (WB). Human tonsil tissue (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ICOSLG

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Alternative Name: ICOSLG

Background: B7 homolog 2 (B7H2), B7 like protein Gl50, B7 related protein 1 (B7RP1), CD275, CD275 antigen, GL50, ICOS LG, ICOS ligand (ICOSL), Inducible T cell co stimulator ligand, LICOS, Transmembrane protein B7 H2 ICOS ligand,ICOS-L / ICOS Ligand / B7RP-1 (Immuno-Oncology Target)
Cellular localisation: Cell Surface and Cytoplasmic

Molecular Weight: 36kDa

Gene ID: 23308, 14155

UniProt: O75144

Pathways: Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation