IGHA1 & IGHA2 antibody

Catalog No. ABIN6937489

The Mouse Monoclonal anti-IGHA1 & IGHA2 antibody (Clone HISA43) (ABIN6937489) specifically detects IGHA1 & IGHA2 in IHC, FACS and IF. The antibody is reactive with Human samples.

Quick Overview for IGHA1 & IGHA2 antibody (ABIN6937489)

Target: IGHA1 & IGHA2

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: HISA43

Product Details

Purpose: IgA (Immunoglobulin Alpha Heavy Chain) (B-Cell Marker) Antibody

Specificity: This MAb is specific to heavy chain of IgA and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with both IgA1 and IgA2 subclasses of Alpha heavy chain. It reacts with the third constant domain (CH3) of the alpha chain of IgA molecules. Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through inter-chain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin A (IgA) is the main protein of the mucosal immune system. It is generated by B-cells in gut-associated lymphoid tissues. Daily production of IgA exceeds that of any of the other immunoglobulins.IgA exists mainly in dimers but can also exist as polymers or as monomers. Dimers and polymers contain a joining (J) chain that can be bound by the polymeric immunoglobulin receptor (pIgR) for transportation of the molecule to mucosal surfaces. The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Purified human IgA

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL 30 min RT), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL 30 min RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Daudi, 293T, Raji or hPBL cells. Tonsil or Spleen.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: IGHA1 & IGHA2

Background:

A2m Marker, Ig alpha 1 Chain C Region, Ig alpha 2 Chain C Region, IGHA1, IGHA2, Immunoglobulin Am1, Immunoglobulin Am2, Immunoglobulin Heavy Constant Alpha 1, Immunoglobulin Heavy Constant Alpha 2

293T, Daudi, Raji or hPBL cells. Tonsil or Spleen.

Molecular Weight: 50-75kDa

Gene ID: 3493, 3494, 699841

UniProt: P01876