IL3RA antibody (AA 26-171)

Catalog No. ABIN6937561

This anti-IL3RA antibody is a Mouse Monoclonal antibody detecting IL3RA in ISt and IHC (f). Suitable for Human.

Quick Overview for IL3RA antibody (AA 26-171) (ABIN6937561)

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL3RA antibody is un-conjugated

Application: Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: IL3RA-1531

Product Details anti-IL3RA Antibody

Binding Specificity: AA 26-171

Specificity: CD123 is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudo-autosomal region on chromosomes X or Y.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human IL3RA protein (around aa 26-171) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: THP-1, RPMI-8226 or HDLM-2 cells. Tonsil, Lymph Node or Stomach.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for IL3RA

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Alternative Name: IL3RA

Background: CD123, hIL3Ra, IL-3R-alpha, IL-3RA, IL3RAX, IL3RAY, IL3RX, IL3RY, Interleukin-3 Receptor, alpha, Interleukin3 receptor, Interleukin3 receptor, Y-chromosomal,IL3RA / CD123 (Acute Myeloid Leukemia Marker)
Cellular localisation: Cell Surface and Cytoplasmic

Molecular Weight: ~70kDa

Gene ID: 3563, 632790

UniProt: P26951

Pathways: JAK-STAT Signaling