CD103 antibody (AA 775-855)

Catalog No. ABIN6937579

The Mouse Monoclonal anti-CD103 antibody (Clone ITGAE-2063) (ABIN6937579) specifically detects CD103 in IHC. The antibody is reactive with Human samples.

Quick Overview for CD103 antibody (AA 775-855) (ABIN6937579)

Target: CD103 (ITGAE) (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD103 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: ITGAE-2063

Product Details anti-CD103 Antibody

Binding Specificity: AA 775-855

Purpose: CD103 / Integrin αEβ7 (T-Cell Lymphoma & Hairy Cell Leukemia Marker) Antibody

Specificity: This MAb recognizes a protein of 150 kDa, identified as CD103, which is the alpha-E integrin subunit of the heterodimeric alpha-E beta-7 (aEb7) integrin belonging to a small beta-7 integrin subfamily. CD103 is expressed on more than 95 % of intraepithelial CD8+ cells and on 40 % of mucosa-associated T cells, whereas less than 2 % of resting blood lymphocytes are CD103-positive. In several malignant conditions, such as T-cell lymphomas and hairy cell leukemia (HCL), the cells express CD103. Antibody to CD103 is an extremely useful addition to the IHC panel for the diagnosis of hairy cell leukemia (HCL).

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human ITGAE/CD103 protein fragment (aa 775-855) (exact sequence is proprietary)

Isotype: IgG2c, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Tonsil, Colon or Stomach.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD103

Target: CD103 (ITGAE) (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))

Alternative Name: ITGAE

Background:

CD103, Human mucosal lymphocyte antigen 1 (HML1 antigen), Integrin alpha E heavy chain, Integrin alpha E light chain, Integrin alpha E, epithelial-associated, Integrin alpha E1, Integrin alpha-IEL (intestinal intraepithelial lymphocytes), Integrin alpha M290, Integrin alpha-E heavy chain, Integrin, alpha E (ITGAE)

Colon or Stomach, Tonsil

Molecular Weight: 150kDa

Gene ID: 3682, 513867

UniProt: P38570