Involucrin antibody

Catalog No. ABIN6937606

The Mouse Monoclonal anti-Involucrin antibody (Clone SY5) (ABIN6937606) specifically detects Involucrin in IHC. The antibody is reactive with Human, Dog, Pig, Gorilla and Owl Monkey samples.

Quick Overview for Involucrin antibody (ABIN6937606)

Target: Involucrin (IVL)

Reactivity: Human, Dog, Pig, Gorilla, Owl Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Involucrin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: SY5

Product Details anti-Involucrin Antibody

Purpose: Involucrin (Squamous Cell Terminal Differentiation Marker) Antibody

Specificity: It recognizes a protein of 66 kDa-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 kDa and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

No Cross-Reactivity: Mouse (Murine)

Cross-Reactivity (Details): Human. Gorilla. Owl monkey. Pig. Dog. Does not react with Mouse.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Purified involucrin from human keratinocytes

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37'C)(Note than enzyme digestion is better than citrate buffer based epitope unmasking); Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 cells. Localized to upper spinous and granular layers in normal skin.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Involucrin

Target: Involucrin (IVL)

Alternative Name: IVL

Background:

IVL

MCF-7 cells. Localized to upper spinous and granular layers in normal skin.

Molecular Weight: 66-170kDa

Gene ID: 3713, 516439

UniProt: P07476