Cytokeratin 1 antibody (C-Term)

Catalog No. ABIN6937639

This anti-Cytokeratin 1 antibody is a Mouse Monoclonal antibody detecting Cytokeratin 1 in IHC (f). Suitable for Human.

Quick Overview for Cytokeratin 1 antibody (C-Term) (ABIN6937639)

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 1 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: LHK1

Product Details anti-Cytokeratin 1 Antibody

Binding Specificity: C-Term

Specificity: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Cytokeratin 1 is a hetero-tetramer of two type I as well as two type II keratins, keratin-10 (KRT10). Keratin 1 is differentiation specific keratin that is one of the predominant keratins in suprabasal keratinocytes in stratified epithelia. Mutations in keratin 1 cause epidermolytic hyperkeratosis.

Cross-Reactivity (Details): Human. Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A 13-amino acid peptide (VRFVSTSYSGVTR) from the C-terminus of human KRT1 protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Skin or Cervix.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Cytokeratin 1

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Alternative Name: KRT1

Background: 67 kDa Cytokeratin, CK1, Cytokeratin-1, EHK1, Epidermolytic hyperkeratosis 1, EPPK, K1, Keratin Type II Cytoskeletal 1, Keratin-1, KRT1, KRT1A, NEPPK, Type II cytoskeletal 1, Type-II keratin Kb1,Cytokeratin 1 (Suprabasal Keratinocyte Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 67kDa

Gene ID: 38748, 80828

UniProt: P04264