KRT15 antibody (C-Term)

Catalog No. ABIN6937660

This anti-KRT15 antibody is a Mouse Monoclonal antibody detecting KRT15 in IHC (fro) and StM. Suitable for Human.

Quick Overview for KRT15 antibody (C-Term) (ABIN6937660)

Target: KRT15 (Keratin 15 (KRT15))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT15 antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Staining Methods (StM)

Clone: SPM190

Product Details anti-KRT15 Antibody

Binding Specificity: C-Term

Specificity: Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. Keratin 15 is down regulated in activated keratinocytes. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.

Cross-Reactivity (Details): Human. Rat. Cow.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A 17 amino acids synthetic peptide from the C- terminal of Human Cytokeratin 15

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Frozen & formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: A431 cells. Skin or Colon.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for KRT15

Target: KRT15 (Keratin 15 (KRT15))

Alternative Name: KRT15

Background: CK15, Cytokeratin 15, K1CO, Ka15, Keratin 15 basic, Keratin 15 beta, Keratin complex 1 acidic gene 15, Keratin type I cytoskeletal 15, KRT15, KRTB, KRTL15, Type I cytoskeletal 15, Type I keratin Ka15,Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 52kDa

Gene ID: 3866, 654570

UniProt: P19012