Recombinant KRT16 antibody (C-Term)

Catalog No. ABIN6937671

The Rabbit Monoclonal anti-KRT16 antibody has been validated for IHC (f). It is suitable to detect KRT16 in samples from Human.

Quick Overview for Recombinant KRT16 antibody (C-Term) (ABIN6937671)

Target: KRT16 (Keratin 16 (KRT16))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This KRT16 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: KRT16-2043R

Product Details anti-KRT16 Antibody

Binding Specificity: C-Term

Specificity: Cytokeratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I Cytokeratins (Cytokeratins 9 to 23) and one type II Cytokeratins (keratins 1 to 8). The cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, to maintain the overall structural integrity of epithelial cells. Cytokeratins are also useful markers in identifying the origin of metastatic tumors. Cytokeratin 16 is expressed in benign stratified squamous epithelium and squamous cell carcinoma of the head and neck, as well as luminal cells of mammary gland and sweat ducts. It is absent in non-invasive breast carcinomas and normal breast tissue.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment from the C-terminal of human Cytokeratin 16

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Tonsil, Skin. Bladder or Cervix.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for KRT16

Target: KRT16 (Keratin 16 (KRT16))

Alternative Name: KRT16

Background: CK16, Cytokeratin-16, Focal non epidermolytic palmoplantar keratoderma (FNEPPK / NEPPK), K1CP, Keratin Type I Cytoskeletal 16, Keratin-16, KRT16A,Cytokeratin 16 (KRT16) (Suprabasal Keratinocyte Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 48kDa

Gene ID: 3868, 655160

UniProt: P08779