KRT4 antibody (AA 181-292)

Catalog No. ABIN6937710

The Mouse Monoclonal anti-KRT4 antibody has been validated for FACS, IF and IHC (f). It is suitable to detect KRT4 in samples from Human.

Quick Overview for KRT4 antibody (AA 181-292) (ABIN6937710)

Target: KRT4 (Keratin 4 (KRT4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: KRT4-2804

Product Details anti-KRT4 Antibody

Binding Specificity: AA 181-292

Specificity: Cytokeratin 4 (KRT4) is an intermediate filament protein associated with Cytokeratin 13 (KRT13). It is expressed in suprabasal cells of non-keratinized stratified squamous epithelium of esophagus, cornea, anus, larynx, pharynx and tongue. Decreased expression of KRT4 is associated with head and neck squamous carcinoma. It is helpful in differentiation of squamous cell carcinoma of esophagus origin from that of thyroid origin.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 181-292) of human KRT4 protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, A549 or A431 cells. Skin, Cervix or Esophagus.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for KRT4

Target: KRT4 (Keratin 4 (KRT4))

Alternative Name: KRT4

Background: CK4, CYK4, Cytokeratin 4, Keratin 4, Keratin type II cytoskeletal 4, KRT4,Cytokeratin 4 (KRT4)
Cellular localisation: Cytoplasmic and Cell Surface

Molecular Weight: 67kDa

Gene ID: 3851, 654610, 706527

UniProt: P19013