KRT6A antibody

Catalog No. ABIN6937715

This Mouse Monoclonal antibody specifically detects KRT6A in FACS and IHC (f). It exhibits reactivity toward Human.

Quick Overview for KRT6A antibody (ABIN6937715)

Target: KRT6A (Keratin 6A (KRT6A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT6A antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: KRT6A-2368

Product Details anti-KRT6A Antibody

Specificity: This MAb recognizes a protein of 56 kDa, identified as cytokeratin 6A (KRT6A). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns. Cytokeratin 6A is the dominant form in epithelial tissue. Cytokeratin 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Cytokeratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express KRT6 and KRT16. KRT6 is strongly expressed in about 75 % of head and neck squamous cell carcinomas..

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human Cytokeratin 6A (KRT6A) protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells, Tonsil or Basal Cell Carcinoma (BCC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for KRT6A

Target: KRT6A (Keratin 6A (KRT6A))

Alternative Name: KRT6A

Background: 58 kDa Cytokeratin, CK5, Cytokeratin-5, DDD1, Epidermolysis Bullosa Simplex 2 (EBS2), Keratin 5, Keratin, Type II Cytoskeletal 5, Keratin-5, KRT5, Type-II Cytoskeletal 5, Type-II keratin Kb5,Cytokeratin 6A (KRT6A) (Basal Cell Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 56kDa

Gene ID: 3853, 700779

UniProt: P02538