KRT6A antibody

Catalog No. ABIN6939867

This Mouse Monoclonal antibody specifically detects KRT6A in IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for KRT6A antibody (ABIN6939867)

Target: KRT6A (Keratin 6A (KRT6A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT6A antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: KRT6A-2368

Product Details anti-KRT6A Antibody

Purpose: Cytokeratin 6A (KRT6A) (Basal Cell Marker) Antibody

Specificity: This MAb recognizes a protein of 56 kDa, identified as cytokeratin 6A (KRT6A). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns. Cytokeratin 6A is the dominant form in epithelial tissue. Cytokeratin 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Cytokeratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express KRT6 and KRT16. KRT6 is strongly expressed in about 75 % of head and neck squamous cell carcinomas..

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human Cytokeratin 6A (KRT6A)protein

Isotype: IgG2a, kappa

Application Details

Application Notes:

Positive Control: HeLa cells, Tonsil or Basal Cell Carcinoma (BCC).

Known Application: Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for KRT6A

Target: KRT6A (Keratin 6A (KRT6A))

Alternative Name: KRT6A

Background:

58 kDa Cytokeratin, CK5, Cytokeratin-5, DDD1, Epidermolysis Bullosa Simplex 2 (EBS2), Keratin 5, Keratin, Type II Cytoskeletal 5, Keratin-5, KRT5, Type-II Cytoskeletal 5, Type-II keratin Kb5

HeLa cells. Human tonsil or basal cell carcinoma (BCC).

Molecular Weight: 56kDa

Gene ID: 3853, 700779

UniProt: P02538