LSP1 antibody (AA 174-278)

Catalog No. ABIN6937790

This Mouse Monoclonal antibody specifically detects LSP1 in IHC (p) and IHC (f). It exhibits reactivity toward Human.

Quick Overview for LSP1 antibody (AA 174-278) (ABIN6937790)

Target: LSP1 (Lymphocyte-Specific Protein 1 (LSP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LSP1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: LSP1-3025

Product Details anti-LSP1 Antibody

Binding Specificity: AA 174-278

Specificity: LSP1 is a hematopoietic-expressed gene that encodes an F-actin-binding, leukocyte-specific (including B and T lymphocytes, granulocytes and macrophages), phosphoprotein. However, mRNA splice variants that do not encode the lympho-specific protein are expressed from this gene in nonlymphoid cell lines as well (myocytes, stromal cells and fibroblasts), suggesting pp52 has a divergent role in signal transduction. The pp52 (LSP1) locus maps to human chromosome 11p15.5, which is implicated in tumor-related chromosomal translocations found in chronic lymphocytic leukemia. The pp52 promoter contains key elements that control transcriptional activity including an initiator specifying the unique 5' terminus of pp52 mRNA, tandem pairs of Ets and SP1 motifs, and a single C/EBP motif. LSP1 binds the cytoskeleton and has been implicated in affecting cytoskeletal remodeling in a variety of leukocyte functions, including cell motility and chemotaxis.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human LSP1 protein (around aa174-278) (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil, colon, spleen or Hodgkin's lymphoma (IHC-P).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for LSP1

Target: LSP1 (Lymphocyte-Specific Protein 1 (LSP1))

Alternative Name: LSP1

Background: WP34, pp52, lymphocyte specific protein 1, leukocyte specific protein 1,Lymphocyte Specific Protein 1 (LSP1) / pp52
Cellular localisation: Membrane.

Molecular Weight: 52kDa

Gene ID: 4046, 56729

UniProt: P33241