Recombinant MLANA antibody

Catalog No. ABIN6937853

This Mouse Monoclonal antibody specifically detects MLANA in WB and IHC (f). It exhibits reactivity toward Human.

Quick Overview for Recombinant MLANA antibody (ABIN6937853)

Target: MLANA (Melan A (MLANA))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLANA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: RMLANA-788

Product Details anti-MLANA Antibody

Specificity: This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Cross-Reactivity (Details): Human. Mouse. Rat. Dog.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length hMART-1 protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: COLO38, SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MLANA

Target: MLANA (Melan A (MLANA))

Alternative Name: MLANA

Background: Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA,MART-1 / Melan-A / MLANA (Melanoma Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 20-22kDa (doublet)

Gene ID: 2315, 154069

UniProt: Q16655