MLANA antibody

Catalog No. ABIN6937860

The Mouse Monoclonal anti-MLANA antibody has been validated for WB, IF, FACS and IHC (f). It is suitable to detect MLANA in samples from Human.

Quick Overview for MLANA antibody (ABIN6937860)

Target: MLANA (Melan A (MLANA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLANA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: A103-M2-7C10-M2-9E3

Product Details anti-MLANA Antibody

Specificity: This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb cocktail labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Cross-Reactivity (Details): Human. Mouse. Rat. Dog.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length hMART-1 protein (A103, M2-7C10, M2-9E3)

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MLANA

Target: MLANA (Melan A (MLANA))

Alternative Name: MLANA

Background: Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA,MART-1 / Melan-A / MLANA (Melanoma Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 20-22kDa (doublet)

Gene ID: 2315, 154069

UniProt: Q16655