Recombinant MVP antibody
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- Target See all MVP Antibodies
- MVP (Major Vault Protein (MVP))
- Antibody Type
- Recombinant Antibody
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This MVP antibody is un-conjugated
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Application
- Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Recognizes a protein of 104 kDa-110 kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for 70 % of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant full-length human MVP protein
- Clone
- VP2897R
- Isotype
- IgG
- Top Product
- Discover our top product MVP Primary Antibody
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- Application Notes
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: MCF-7 or HeLa cells. Breast tumors.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- MVP (Major Vault Protein (MVP))
- Alternative Name
- MVP (MVP Products)
- Synonyms
- LRP antibody, VAULT1 antibody, 2310009M24Rik antibody, cb771 antibody, wu:fb52b05 antibody, wu:fc02g01 antibody, mvp antibody, MGC145641 antibody, Tb05.45E22.810 antibody, major vault protein antibody, major vault protein L homeolog antibody, putative major vault protein antibody, MVP antibody, Mvp antibody, mvp antibody, mvp.L antibody, Tc00.1047053510353.10 antibody, Tb927.5.4460 antibody, Tb10.70.0520 antibody, Tb10.70.5840 antibody, LMJF_05_0060 antibody
- Background
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Lung resistance-related protein (LRP), Major Vault Protein, MVP, VAULT1,Major Vault Protein (MVP)
Cellular localisation: Cytoplasmic - Molecular Weight
- 104-110kDa
- Gene ID
- 9961, 632177
- UniProt
- Q14764
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