MYCN antibody

Catalog No. ABIN6937987

The Mouse Monoclonal anti-MYCN antibody (Clone NMYC-1) (ABIN6937987) specifically detects MYCN in WB and IF. The antibody is reactive with Human samples.

Quick Overview for MYCN antibody (ABIN6937987)

Target: MYCN (N-Myc Proto-Oncogene Protein (MYCN))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYCN antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: NMYC-1

Product Details anti-MYCN Antibody

Specificity: The v-Myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans, the family consists of five genes: c-Myc, N-Myc, R-Myc, L-Myc and B-Myc. Amplification of the N-Myc gene has been found in human neuroblastomas and cell lines. Its amplification correlates well with the stage of neuroblastoma disease. Immunological studies have shown that the human N-Myc gene encodes a nuclear phosphoprotein that exhibits relatively short (30 min) half life in vivo. The prototype member of the family, c-Myc p67, binds DNA in a sequence-specific manner subsequent to dimerization with a second basic region helix-loop-helix leucine zipper motif protein, designated Max.

Cross-Reactivity (Details): Human. Mouse. Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human n-Myc protein.

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: 293T whole cell lysates or recombinant Human n-Myc protein.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MYCN

Target: MYCN (N-Myc Proto-Oncogene Protein (MYCN))

Alternative Name: MYCN

Background: Class E basic helix-loop-helix protein 37 (bHLHe37), MODED, N-myc proto-oncogene protein, Neuroblastoma derived v myc avian myelocytomatosis viral related oncogene, ODED, pp65/67, v myc (avian) myelocytomatosis viral related oncogene neuroblastoma derived,n-Myc (Neuroblastoma Marker)
Cellular localisation: Nucleus.

Molecular Weight: 67kDa

Gene ID: 4613, 25960

UniProt: P04198