NEFH antibody

Catalog No. ABIN6938031

The Mouse Monoclonal anti-NEFH antibody has been validated for FACS and IHC (f). It is suitable to detect NEFH in samples from Rat.

Quick Overview for NEFH antibody (ABIN6938031)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFH antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: RT97

Product Details anti-NEFH Antibody

Specificity: This MAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Cross-Reactivity (Details): Human. Mouse. Rat. Chicken. Pig.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Triton-X 100 insoluble protein fraction of rat brain

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Brain, Neuroblastoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Alternative Name: NEFH

Background: NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200 kDa, Neurofilament Triplet H Protein, NF-H, NF200,Neurofilament (NF-H) (Neuronal Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 200kDa

Gene ID: 4744, 198760

UniProt: P12036