NKX2-1 antibody

Catalog No. ABIN6938054

This Mouse Monoclonal antibody specifically detects NKX2-1 in FACS, IF and IHC (f). It exhibits reactivity toward Rat.

Quick Overview for NKX2-1 antibody (ABIN6938054)

Target: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NKX2-1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 8G7G3-1

Product Details anti-NKX2-1 Antibody

Specificity: Recognizes a protein of 40 kDa, identified as Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates.Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.

Cross-Reactivity (Details): Human. Mouse. Rat. Shows a broad species reactivity.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Rat full length TTF-1 recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MAD109, MLE-15, H441-4 or H345 cells. Normal thyroid or lung.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for NKX2-1

Target: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Alternative Name: NKX2-1

Background: BCH, Benign chorea, BHC, Homeobox protein Nkx2.1, NK2 homeobox 1, NKX2.1, NKX2A, TEBP, Thyroid nuclear factor-1, Thyroid specific enhancer binding protein, Thyroid transcription factor-1 (TTF-1), Tin man, TITF1, TTF-1,TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker)
Cellular localisation: Nuclear

Molecular Weight: 40kDa

Gene ID: 7080, 94367

UniProt: P43699

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Glucan Metabolic Process, Feeding Behaviour