NME2 antibody

Catalog No. ABIN6938079

The Mouse Monoclonal anti-NME2 antibody has been validated for WB and IHC (f). It is suitable to detect NME2 in samples from Human.

Quick Overview for NME2 antibody (ABIN6938079)

Target: NME2 (Nucleoside diphosphate kinase B (NME2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NME2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CPTC-NME2-2

Product Details anti-NME2 Antibody

Specificity: The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human c-Myc promotor.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human NME2 protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: PC3, A549, HeLa, Jurkat cells. Ubiquitously expressed in all tissues.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for NME2

Target: NME2 (Nucleoside diphosphate kinase B (NME2))

Alternative Name: NME2

Background: C myc purine binding transcription factor PUF, C-myc purine-binding transcription factor PUF, epididymis secretory sperm binding protein Li 155an, HEL-S-155an, Histidine protein kinase NDKB, NDP kinase B, NDPKB, nm23-H2, NM23B, NME/NM23 nucleoside diphosphate kinase 2, nme2, Nucleoside diphosphate kinase B,NME2 / nm23-H2 / NDPK-B (Suppressor of Metastasis)
Cellular localisation: Cytoplasmic and Nuclear

Molecular Weight: 17kDa

Gene ID: 4831, 463456

UniProt: P22392