Nuclear Antigen (Pan-Nuclear Marker) antibody

Catalog No. ABIN6938094

The Mouse Monoclonal anti- antibody has been validated for IHC, ISt, StM, FACS and IF. It is suitable to detect in samples from Human, Mouse and Rat.

Quick Overview for Nuclear Antigen (Pan-Nuclear Marker) antibody (ABIN6938094)

Target: Nuclear Antigen (Pan-Nuclear Marker)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: NM106

Product Details

Specificity: This MAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

No Cross-Reactivity: Pig (Porcine)

Purification: Purified by Protein A/G

Immunogen: Nuclei of HL60 cells

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: All human cells. Tonsil.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Buffer: 10 mM PBS without BSA and without Azide.

Preservative: Azide free

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Expiry Date: 24 months

Target Details

Target: Nuclear Antigen (Pan-Nuclear Marker)